3rd Lublin International Medical Congress for Students and..

Conference Workshops

Short Course in Molecular Biology: PCR Basic Course

Short Course in Molecular Biology: PCR Basic Course

 

LECTURER: dr n. farm. Izabela Korona-Głowniak

 

Duration: 2 hours

Location: Department of Pharmaceutical Microbiology, Collegium Universum, I floor

 

The purpose of this course is to learn the principles and applications of the Polymerase Chain Reaction in microbiological diagnostics.

 

The theoretical part includes:

* PCR basics

* Optimization of PCR parameters

* Trouble shooting

The practical part includes:

* Isolation of genomic DNA

* Set up of various PCR reactions, e.g. multiplex PCR

* Detection of PCR products by means of gel electrophoresis

* Analysis of results

11 December 2015

I 12:00 PM – 02:00 PM (5 people)

II 02:00 PM -04:00 PM (5 people)

 

12 December 2015

I 9:00 AM – 9:30 AM (5 people)

II 9:00 AM – 9:30 AM (5 people)

Flow Cytometry Course

Flow Cytometry Course

 

LECTURERS: dr hab. Agnieszka Bojarska-Junak

dr hab. Piotr Pożarowski

 

Duration: 5 hours

Location: Chair and Department of Clinical Immunology, Collegium Pharmaceuticum, I floor

 

The purpose of this course is to learn the principles and applications of flow cytometry.

 

Topics discussed at the workshop:

1. Surface marker analysis by flow cytometry (dr hab. Agnieszka Bojarska-Junak)

2. Detection of intracellular cytokines (dr hab. Agnieszka Bojarska-Junak)

3. Analysis of DNA by propidium iodide and acridine orange (dr hab. Piotr Pożarowski)

4. Detection of apoptosis (dr hab. Piotr Pożarowski)

 

12 December 2015

I 10:00 AM – 12:30 PM (dr hab. Agnieszka Bojarska-Junak) (6 people)

II 10:00 AM – 12:30 PM (dr hab. Piotr Pożarowski) (6 people)

 

I 12:30 PM – 03:00 PM (dr hab. Piotr Pożarowski) (6 people)

II 12:30 PM – 03:00 PM (dr hab. Agnieszka Bojarska-Junak) (6 people)

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Calendar

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